Morphine induces changes in astrocyte transcriptional dynamics in the ventral tegmental area through glucocorticoid signaling.

Morphine regulates astrocyte transcriptional dynamics in the ventral tegmental area by stimulation of glucocorticoid signaling hypothes.is/a/BKNk7D4mEfGSr3euTP2NkQ **Reviewer #1 (Public review):** Studies investigating global gene expression changes induced by a single morphine administration have previously been conducted in several rodent brain regions. In this work, the authors focused on the ventral…

Morphine regulates astrocyte transcriptional dynamics in the ventral tegmental area by stimulation of glucocorticoid signaling hypothes.is/a/BNGaJD4mEfGY2xMDcbl3nw **eLife Assessment** This important study examines how chronic pain and opioid exposure interact at the cellular and molecular levels in a reward-related brain region. Using single-nucleus RNA sequencing, the authors map transcriptional changes in the…

Morphine regulates astrocyte transcriptional dynamics in the ventral tegmental area by stimulation of glucocorticoid signaling hypothes.is/a/BHHHrD4mEfGqBjP4OSFqjA **Reviewer #2 (Public review):** Summary: This study addresses an important gap in our understanding of how pain‑related neuroadaptations interact with opioid exposure at the cellular and molecular levels, particularly in terms of…

Morphine regulates astrocyte transcriptional dynamics in the ventral tegmental area by stimulation of glucocorticoid signaling hypothes.is/a/BEAH2j4mEfGNBe-_T96-pA **Reviewer #3 (Public review):** Summary: This work examined the transcriptional response to pain induction by CFA and/or morphine treatment in rat VTA at the level of single cells. This builds on prior work using bulk-tissue RNA-seq to evaluate…