The bifunctional architecture of TDM enables both substrate catalysis and channeling in Paracoccus.

Bifunctional Architecture Enables Substrate Catalysis and Channeling in Paracoccus TMAO Demethylase hypothes.is/a/I6V0YD42EfGSxpsmrJTBeQ **Reviewer #1 (Public review):** Summary: Thach et al. report on the structure and function of trimethylamine N-oxide demethylase (TDM). They identify a novel complex assembly composed of multiple TDM monomers and obtain high-resolution…

Bifunctional Architecture Enables Substrate Catalysis and Channeling in Paracoccus TMAO Demethylase hypothes.is/a/IzkEVj42EfGQSv_ZtIkm_w **Author response:** The following is the authors’ response to the original reviews. > **Public Reviews:** > > **Reviewer #1 (Public review):** > > Summary: > > Thach et al. report on the structure and function of trimethylamine N-oxide demethylase…

Bifunctional Architecture Enables Substrate Catalysis and Channeling in Paracoccus TMAO Demethylase hypothes.is/a/I3QLbj42EfGsDdsxlOe5IQ **Reviewer #2 (Public review):** Summary: The manuscript reports a cryo-EM structure of TMAO demethylase from Paracoccus sp. This is an important enzyme in the metabolism of trimethylamine oxide (TMAO) and trimethylamine (TMA) in human gut…