The UniDesign computational framework successfully engineered a PAM-relaxed Staphylococcus aureus Cas9 variant with expanded targeting capabilities.

Fully computational design of PAM-relaxed Staphylococcus aureus Cas9 with expanded targeting capability using UniDesign hypothes.is/a/lvG0Dj2YEfGyqbNSoOdO0Q **eLife Assessment** This important study demonstrates the power of the UniDesign computational framework in prospectively engineering a PAM-relaxed Staphylococcus aureus Cas9 variant with editing performance comparable to evolution-derived…

Fully computational design of PAM-relaxed Staphylococcus aureus Cas9 with expanded targeting capability using UniDesign hypothes.is/a/lisXBD2YEfGUAJdgoYgxfA **Author response:** The following is the authors’ response to the original reviews. > **Public Reviews:** > > **Reviewer #1 (Public review):** > > Summary: > > This manuscript by Xiong and colleagues presents a compelling validation of UniDesign, a…

Fully computational design of PAM-relaxed Staphylococcus aureus Cas9 with expanded targeting capability using UniDesign hypothes.is/a/lr2mZD2YEfGloJeeDlT-5g **Reviewer #1 (Public review):** \[Editors' note: The Reviewing Editor has assessed the work without involving the previous reviewers, updating the eLife Assessment accordingly. The authors did an excellent job of addressing the reviewers' comments and…